Proteomics Technology Core Facility
Summary
The Proteomics Technology Core Facility (PTCF) initiative established facilities at nine institutions in three regions - western, central, and eastern - across southern Ontario to identify proteins and characterize them by mass spectrometry:
- Western: McMaster University, University of Guelph, and University of Western Ontario
- Central: Hospital for Sick Children, Samuel Lunenfeld Research Institute (Mount Sinai Hospital), University of Toronto, and York University
- Eastern: Queen’s University and University of Ottawa
The institutions supported Genome Canada-funded projects and many other projects and collaborations, and provided fee-based services to corporations and university-based investigators.
Fast Facts
- Highlighted outcome: All nine facilities have become valuable resources for proteomics analyses.
- Resources generated: Two significant proteomics databases.
- Number of patents in process or obtained: 8.
Notable Publications
Schuldiner M, Collins SR, Thompson NJ, Denic V, Bhamidipati A, Punna T, Ihmels J, Andrews B, Boone C, Greenblatt JF, Weissman JS, and Krogan NJ. 2005. Exploration of the function and organization of the yeast early secretory pathway through an epistatic miniarray profile. Cell 3:507-19.
Keogh MC, Kurdistani SK, Keogh MC, Kurdistani SK, Morris SA, Ahn SH, Podolny V, Collins SR, Schuldiner M, Chin K, Punna T, Thompson NJ, Boone C, Emili A, Weissman JS, Hughes TR, Strahl BD, Grunstein M, Greenblatt JF, Buratowski S, and Krogan NJ. 2005. Cotranscriptional set2 methylation of histone H3 lysine 36 recruits a repressive Rpd3 complex. Cell 4:593-605.
Keogh MC, Kim JA, Downey M, Fillingham J, Chowdhury D, Harrison JC, Onishi M, Datta N, Galicia S, Emili A, Lieberman J, Shen X, Buratowski S, Haber JE, Durocher D, Greenblatt JF, and Krogan N. 2005. A phosphatase complex that dephosphorylates gammaH2AX regulates DNA damage checkpoint recovery. Nature 7075: 497-501.
Zhao R, Davey M, Hsu YC, Kaplanek P, Tong A, Parsons AB, Krogan N, Cagney G, Mai D, Greenblatt J, Boone C, Emili A, and Houry WA. 2005. Navigating the chaperone network: an integrative map of physical and genetic interactions mediated by the hsp90 chaperone. Cell 5:715-27.
